logo
Volume 8, Issue 24 (Spring 2025)                   J Altern Vet Med 2025, 8(24): 20-29 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Zarei P, Shafiei E, Yazdanpanah I, Hoseinzadeh M, Zarei P. The Sero-epidemiological Investigation of Brucellosis in Cows and Sheep in Firozabad city, Fars, Iran: A Cross-Sectional Study. J Altern Vet Med 2025; 8 (24) :20-29
URL: http://joavm.kazerun.iau.ir/article-1-165-en.html
The Sero-epidemiological Investigation of Brucellosis in Cows and Sheep in Firozabad city, Fars, Iran: A Cross-Sectional Study
Pouria Zarei *1 , Elham Shafiei2 , Iynaz Yazdanpanah2 , Mohammdhossein Hoseinzadeh2 , Pedram Zarei
1- Department of Parasitology, Collage of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran , pouriazarei95@gmail.com
2- Department of Parasitology, Collage of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Abstract:   (42 Views)

Background and aim: Brucellosis is considered as one the bacterial zoonoses which is associated with abortions and infertility in cattle and sheep especially in developing countries. Among the different species of the genus Brucella, B. abortus is the most common species infects cattle globally, whereas B. melitensis mainly infects on sheep. The risk of brucellosis transmission (Malta fever) to consumers due to the existence of nomads and traditional livestock production and farming in Firozabad city, Fars province, Iran could be assumed high. Therefore, the recent study aims to determine the Sero-epidemiological investigation of brucellosis in cows and sheep in Firozabad city, Fars, Iran.
Materials and Methods: 200 blood samples were collected during 5 months, categorized based on species, sexuality, age, and type of maintenance. The samples were subjected to the Rose Bengal test, Wright, and 2-Mercaptoethanol (2-ME) tests. The results were statistically analyzed using SPSS software and the t-test statistical method.
Results: In the Rose Bengal test, out of the total of 200 animal serum samples, 10 samples (5%) were positive (cattle (4.16%)/sheep (6.25%)). The total of 8 cases (4%) were positive in the Wright test (cattle (3.33%)/ sheep (5%)) and in the 2ME test, out of 8 positive cases in the Wright test, the total of 7 cases (3.5%) were positive (cattle (2.5%)/ sheep (5%)). The prevalence of brucellosis is 4% (0.5% cattle+3.5% sheep) and the percentage of infection is 5.15% in female cattle, 0% in bulls, 7.40% in female sheep, and 3.84% in male sheep. In terms of age, infected cows and sheep under one-year are 2.38% and 0%, in over one year sheep and cows’ cases are 8.33% and 5.12% respectively. Finally, the prevalence in traditional farms (7.75%) compared to industrial farms (1.19%) is significantly higher.
Conclusion: The statistical analysis shows that the prevalence of brucellosis in sheep was higher than cows and there was a significant association between brucellosis infection and the gender, age of animals and a significant relationship between the type of maintenance and the percentage of disease is observed. Also, the prevalence rate was higher in traditional farms than in industrial farms.

Keywords: Giardia duodenalis, Cattle, Firozabad city, Iran
Full-Text [PDF 1412 kb]   (14 Downloads)    
Type of Study: Research | Subject: Parasitology
Received: 2024/11/7 | Accepted: 2025/01/4 | Published: 2025/05/31
References
1. Al-Majali AM, Talafha AQ, Ababneh MM, Ababneh MM. Seroprevalence and risk factors for bovine brucellosis in Jordan. J Vet Sci. 2009; 10(1): 61-5. [DOI:10.4142/jvs.2009.10.1.61] [PMID] []
2. Adabi M, Khazaiee S, Sadeghi-Nasab A, Alamian S, Arabestani MR, Valiei Z, et al. Brucellosis in livestock: First study on seroepidemiology, risk factors, and preventive strategies to manage the disease in Famenin, Iran. Vet World. 2022; 15(8): 2102. [DOI:10.14202/vetworld.2022.2102-2110] [PMID] []
3. Aghamohammad S, Rastin M, Mostafavi E, Anaraki AH, Rahravani M, Sadaf RA, et al. Determination of seroprevalence of brucellosis in livestock and high-risk population in Kurdistan, Western Iran. CIMID. 2023; 93: 101942. [DOI:10.1016/j.cimid.2022.101942] [PMID]
4. Akbarmehr J, Ghiyamirad M. Serological survey of brucellosis in livestock animals in Sarab City (East Azarbayjan province), Iran. Afr J Microbiol Res. 2011; 5(10): 1220-3.. [DOI:10.5897/AJMR11.180]
5. Alamian S, Amiry K, Bahreinipour A, Etemadi A, Tebianian M, Mehrabadi MH, et al. Brucella species circulating in rural and periurban dairy cattle farms: a comparative study in an endemic area. Trop Anim Health Prod. 2021; 53(2): 200. [DOI:10.1007/s11250-021-02645-y] [PMID]
6. Arif S, Thomson PC, Hernandez-Jover M, McGill DM, Warriach HM, Heller J. Knowledge, attitudes and practices (KAP) relating to brucellosis in smallholder dairy farmers in two provinces in Pakistan. PloS one. 2017; 12(3): e0173365. [DOI:10.1371/journal.pone.0173365] [PMID] []
7. Bahonar A, Bahreinipour A, Rahimi Forushani A, Lotfolah Zadeh S, Amiri K, Naguibi SB. The status of brucellosis infection in industrial and semi-industrial cattle farms covered by brucellosis testing and killing operations of the country's veterinary organization in 2017. Iranian Journal of Infectious Diseases and Tropical Medicine [Internet]. 2018; 24(86): 7-15. Available from: https://sid.ir/paper/370736/fa
8. Çelebi G, Külah C, Kiliç S, Üstündağ G. Asymptomatic Brucella bacteraemia and isolation of Brucella melitensis biovar 3 from human breast milk. Scand J Infect Dis. 2007; 39(3): 205-8. [DOI:10.1080/00365540600978898] [PMID]
9. Currò V, Marineo S, Vicari D, Galuppo L, Galluzzo P, Nifosì D, et al. The isolation of Brucella spp. from sheep and goat farms in Sicily. Small Rumin Res. 2012; 106: S2-5. [DOI:10.1016/j.smallrumres.2012.04.025]
10. DelVecchio VG, Kapatral V, Redkar RJ, Patra G, Mujer C, Los T, et al. The genome sequence of the facultative intracellular pathogen Brucella melitensis. PNAS. 2002; 99(1): 443-8. [DOI:10.1073/pnas.221575398] [PMID] []
11. Garcell HG, Garcia EG, Pueyo PV, Martín IR, Arias AV, Serrano RN. Outbreaks of brucellosis related to the consumption of unpasteurized camel milk. Journal of infection and public health. 2016; 9(4): 523-7. [DOI:10.1016/j.jiph.2015.12.006] [PMID]
12. Garofolo G, Fasanella A, Di Giannatale E, Platone I, Sacchini L, Persiani T, et al. Cases of human brucellosis in Sweden linked to Middle East and Africa. BMC Res Notes. 2016; 9(1): 277. [DOI:10.1186/s13104-016-2074-7] [PMID] []
13. Gharekhani J, Rasouli M, Abbasi-Doulatshahi E, Bahrami M, Hemati Z, Rezaei A, et al. Sero-epidemiological survey of brucellosis in small ruminants in Hamedan province, Iran. J Adv Vet Anim Res. 2016; 3(4): 399-405. [DOI:10.5455/javar.2016.c179]
14. Gholizadeh SS, Zali MH, Hashempour A, Ahmadi EM. Investigation of brucellosis in cattle and sheep in Urmia-Iran. YYU Veteriner Fakultesi Dergisi. 2013; 24(3): 133-134.
15. Hajibemani A. Seroprevalence of brucellosis in sheep in Tabriz, Iran. NFVM. 2023; 5(2): 1-0.
16. Kaboli Boroujeni H, Abutalebi MS, Kheirollahi N, Haghighat S. Epidemiological surveillance of livestock brucellosis in Borujen county during 2010-2016. 2020; 14(1): 41-50.
17. Khan MZ, Zahoor M. An overview of brucellosis in cattle and humans, and its serological and molecular diagnosis in control strategies. Trop Med Infect Dis. 2018; 3(2): 65. [DOI:10.3390/tropicalmed3020065] [PMID] []
18. Köse Ş, SENGER SS, Akkoçlu G, Kuzucu L, Ulu Y, Ersan G, et al. Clinical manifestations, complications, and treatment of brucellosis: evaluation of 72 cases. Turk J Med Sci. 2014; 44(2): 220-3. [DOI:10.3906/sag-1112-34] [PMID]
19. Mombenı E, Mombeını M, Khalaj M, Asadı R, Rezaeı A, Amırı K, et al. Seroprevalence of brucellosis in livestock in Khuzestan province, southwest of Iran, 2008-2012. Istanbul Univ Vet Fak Derg. 2014; 40(2): 139-46.
20. Narnaware SD, Dahiya SS, Kumar S, Tuteja FC, Nath K, Patil NV. Pathological and diagnostic investigations of abortions and neonatal mortality associated with natural infection of Brucella abortus in dromedary camels. Comp Clin Path. 2017; 26(1): 79-85. [DOI:10.1007/s00580-016-2348-4]
21. Omer MK, Skjerve E, Holstad G, Woldehiwet Z, Macmillan AP. Prevalence of antibodies to Brucella spp. in cattle, sheep, goats, horses and camels in the State of Eritrea; influence of husbandry systems. Epidemiol Infect. 2000; 125(2): 447-453. [DOI:10.1017/S0950268899004501] [PMID] []
22. Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. Lancet Infect Dis. 2006; 6(2): 91-9. [DOI:10.1016/S1473-3099(06)70382-6] [PMID]
23. Rossetti CA, Arenas-Gamboa AM, Maurizio E. Caprine brucellosis: A historically neglected disease with significant impact on public health. PLoS Negl Trop Dis. 2017; 11(8): e0005692. [DOI:10.1371/journal.pntd.0005692] [PMID] []
24. Sánchez DO, Zandomeni RO, Cravero S, Verdún RE, Pierrou E, Faccio P, et al. Gene discovery through genomic sequencing of Brucella abortus. IAI. 2001; 69(2): 865-8. [DOI:10.1128/IAI.69.2.865-868.2001] [PMID] []
25. Selim A, Attia K, Ramadan E, Hafez YM, Salman A. Seroprevalence and molecular characterization of Brucella species in naturally infected cattle and sheep. Prev Vet Med. 2019; 171: 104756. [DOI:10.1016/j.prevetmed.2019.104756] [PMID]
26. Semironi MM, Bahrani M, Mehrabadi MH, Ranjbar N, Absalanfard K, Makan MT, et al. Seroprevalence of brucellosis in rural animals of Bushehr Province, Iran during 2012-2014. 2018; 13(4): 337-345.
27. Shahbazi Y, Afshari Safavi EA, Shavisi N. The epidemiological survey of animal brucellosis in Kermanshah province. IJVCS. 2016; 10(1): e10228.
28. Sharifi H, Mashayekhi K, Tavakoli MM. Risk facts of small ruminant brucellosis: a cross-sectional study in Southeast Iran 2012. Human and Veterinary Medicine. 2015; 7(1): 42-5.
29. Soleimanzadeh A, Jalilzadeh-Amin G, Vaghar A. Prevalence of Brucella abortus in Holstein dairy cows in north west of Iran by using serological and PCR assays. Veterinary Research & Biological Products. 2017; 30(3): 85-91.
30. Wernery U. Camelid brucellosis: a review. Rev sci tech. 2014; 33(3): 839-57. [DOI:10.20506/rst.33.3.2322] [PMID]
31. Zarei P, Shafiei E, Torabi Y, Zarei P, Dehbozorgi R, Kazerun I. The prevalence of giardia duodenalis in dairy calves and cattle in Firozabad city, Iran. J Altern Vet Med. 2024; 7 (22): 1301-1307. [DOI:10.61186/joavm.7.22.1301]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.