Volume 3, Issue 7 (Winter 2020)
J Altern Vet Med 2020, 3(7): 401-413 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Emami A, Khedmati S R. Evaluation of the uncommon bacterial pathogens in urogenital infection of patients referred to Iran Hospital with phenotypic and molecular methods. J Altern Vet Med 2020; 3 (7) :401-413
URL: http://joavm.kazerun.iau.ir/article-1-25-en.html
URL: http://joavm.kazerun.iau.ir/article-1-25-en.html
1- Assistant Professor, Department of Microbiology, Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Iran
2- Master of Microbiology, Islamic Azad University of shiraz, Iran ,s.reza.khedmati@Gmail.com
2- Master of Microbiology, Islamic Azad University of shiraz, Iran ,
Abstract: (287 Views)
Background and aim: One of the most common infections, especially nosocomial infections in humans, is urinary tract infection (UTI). UTI-causing agents include bacteria, fungi and viruses. Bacteria, especially Escherichia coli with about 85% is the most common cause of this type of infection. Other bacteria include Klebsiella, Enterococcus, Pseudomonas, Staphylococcus, Proteus, Neisseria, Mycoplasma and Chlamydia. The aim of this study is evaluation the prevalence of uncommon bacteria in the incidence of UTI using phenotypic and molecular (PCR) methods detection.
Materials and methods: In this study, during six months, 235 urine samples were collected from patients referred to Iran Hospital who had UTI symptoms, and simultaneously with microbial culture in the laboratory, DNA extraction was performed using extraction kits and PCR test with specific primers to detect these bacteria in each Samples were taken.
Results: In the samples, 137 samples (58.29%) contained uncommon UTI bacteria. The prevalence of uncommon bacteria was as follows: Pseudomonas aeruginosa 74 samples (31.4%), Enterococcus faecalis 61 samples (25.9%), Klebsiella pneumoniae 32 samples (13.6%), Staphylococcus saprophyticus 2 Sample (10.2%), Proteus Myrabilis 13 specimens (5.5%), Neisseria gonorrhoea 7 specimens (2.9%), Chlamydia trachomatis and Mycoplasma 5 specimens each (2.1%). Only 19.7% of samples containing uncommon bacteria were detected by routine laboratory methods (phenotypic diagnosis).
Conclusion: The prevalence of these bacteria varies according to the geographical location, level of health, health control and level of culture of each country. Delay in accurate identification of these bacteria increases bacterial resistance to antibiotics prescribed by physicians.
Materials and methods: In this study, during six months, 235 urine samples were collected from patients referred to Iran Hospital who had UTI symptoms, and simultaneously with microbial culture in the laboratory, DNA extraction was performed using extraction kits and PCR test with specific primers to detect these bacteria in each Samples were taken.
Results: In the samples, 137 samples (58.29%) contained uncommon UTI bacteria. The prevalence of uncommon bacteria was as follows: Pseudomonas aeruginosa 74 samples (31.4%), Enterococcus faecalis 61 samples (25.9%), Klebsiella pneumoniae 32 samples (13.6%), Staphylococcus saprophyticus 2 Sample (10.2%), Proteus Myrabilis 13 specimens (5.5%), Neisseria gonorrhoea 7 specimens (2.9%), Chlamydia trachomatis and Mycoplasma 5 specimens each (2.1%). Only 19.7% of samples containing uncommon bacteria were detected by routine laboratory methods (phenotypic diagnosis).
Conclusion: The prevalence of these bacteria varies according to the geographical location, level of health, health control and level of culture of each country. Delay in accurate identification of these bacteria increases bacterial resistance to antibiotics prescribed by physicians.
Type of Study: Research |
Subject:
Microbiology
Received: 2020/07/1 | Accepted: 2020/10/14 | Published: 2020/12/21
Received: 2020/07/1 | Accepted: 2020/10/14 | Published: 2020/12/21
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |